The TANBead Environmental Microbiome DNA kit (6EM series) is an accurate, methodical, and user-friendly environmental microbial extraction reagent used for the rapid and effective isolation and purification of microbial DNA, such as fungi, bacteria, yeast, algae, etc., from various environmental samples like soil, wastewater, and air.
Soil is most common in environmental microbiome samples. The diverse composition, complex microbial interactions, and the presence of inhibitory compounds make extracting microbial total DNA with high-quality a challenging task. Now, the TANBead research team has developed the solutions for two major targeted challenges in nucleic acid purification from soil samples: the difficult microbial lysis and the high humic acid content.
● Efficient Microbial Lysis:
The 6EM kit employs a Beads Beating technique combined with effective chemical reagents, which helps disperse soil particles, increasing the likelihood of collisions between microbes and lysing beads. This results in efficient lysis of Gram-positive bacteria and fungi, and ensures the thorough lysis of all bacteria and fungi present in soil samples.
● Humic Acid Removal:
One of the greatest challenges in DNA extraction from soil samples is the high content of humic substances (humic acids, fulvic acids, etc.). These humic acid molecules contain carboxyl and phenol groups, varying in molecular size, and are similar to nucleic acids as large, hydrophilic anionic polymers, making them prone to co-extraction with nucleic acids in the final steps. The TANBead research team has developed an innovative method specifically designed for environmental samples with a high humic acid content, such as compost, sediment, and fertilizer. After processing, high-quality DNA can be obtained, perfectly suited for downstream PCR, qPCR, and NGS applications.