核酸萃取試劑
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OptiPure Blood DNA (61E)
- HLA-typing, genomic DNA - Fresh blood, frozen blood, or buffy coat - 250~300 ul sample volume - Enhanced lysis with pretreatment of proteinase K
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Tissue DNA (612)
- Genomic DNA - Cultured cells and animal tissue - No pretreatment required - 20-50 mg tissue or 2x10 6 cells
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Tissue Total DNA (6T2)
- HPV screening - Total DNA - Enhanced lysis of animal tissues - 50-100 mg tissues - Incubation buffer+proteinase K at 56℃ for 30~60 mins
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Tissue RNA (6K2)
- RNA expression analysis - Shrimp/fish tissue virus - Extract tissues and virus nucleic acids - No pretreatment required - 30-100 mg animal tissue or 2x10 5 cells
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FFPE DNA (61P)
- Cancer management - DNA extraction from 5-60 um thick FFPE tissue sections - Pretreatment of mineral oil followed by proteinase K treatment
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Plant DNA (613)
- Various plant tissues such as plant and fungi tissues - 20-50 mg plant and fungi tissues - Incubation at RT for 10 mins. Samples with high silicon contents can be pretreated with CTAB buffer
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Rice DNA (619)
- Specifically developed for genetic diversity of rice - 1 grain of rice accessible - Integrated pretreatment by physical disruption in the presence of proteinase K
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Plant RNA (6K3)
- RNA from plant tissue, such as Orchid virus detection - Extract plant and virus nucleic acids - 30-100 mg plant tissue - Incubation with lysis buffer at RT for 10 mins
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Gram Bacteria DNA (61G)
- Enteric microflora analysis/ Food microorganisms - Gram positive and Gram negative bacteria - 1-4 ml pellet from bacterial culture - Incubation with Lysozyme and proteinase K at 60℃ for 20 mins
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Bacterial Plasmid DNA (61H)
- Bacterial plasmid DNA - 1-4 ml pellet from bacterial culture - Incubation with Lysozyme and proteinase K at 60℃ for 5 mins
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Virus DNA - HBV (615)
- Specific for HBV - 300-600 ul serum - Pretreatment of proteinase K at 56℃ for 10 mins
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Viral RNA (635)
- Enterovirus detection - 200-400 ul serum, for most RNA virus - Integrated treatment of proteinase K
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OptiPure Viral Auto Plate/Auto Tube (665)
- HBV, HCV, avian influenza virus detection, and other RNA viruses - 300-600 ul serum - Integrated treatment of proteinase K
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OptiPure cfDNA (61C)
- cfDNA specificity - 1.2 ml plasma, bronchoalveolar lavage fluid (BAL) - Integrated treatment of proteinase K
