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Tissue Total DNA Auto Plate/Auto Tube (6T2) is dedicated to the isolation of DNA from tissues that are difficult to be lysed. Samples need to be first treated with proteinase K, followed by adding samples into Auto Plate/Auto Tube and processed by TANBead’s automation. The automated process is simple and can handle multiple samples at the same time, in contrast to complicated manual extraction processing. The protocol dramatically reduces experimental time and enhances consistency and reproducibility of DNA isolation and is suitable for laboratories with large quantity of samples.